Enzyme information

15 enzymes were used in this database. The cleavage position is between P1 and P1'.

Enzyme cleavage rule

RuleID EnzID EnzName P4 P3 P2 P1 P1' P2' P3'
1 1 Arg-C Proteinase [R]
2 2 Asp-N Endopeptidase NULL [D]
3 3 Chymotrypsin-High specificity [FY] [^P]
4 3 Chymotrypsin-High specificity [W] [^MP]
5 4 Chymotrypsin-Low specificity [FLY] [^P]
6 4 Chymotrypsin-Low specificity [W] [^MP]
7 4 Chymotrypsin-Low specificity [M] [^PY]
8 4 Chymotrypsin-Low specificity [H] [^DMPW]
9 5 Clostripain [R]
10 6 Enterokinase [DE] [DE] [DE] [K]
11 7 Glutamyl Endopeptidase [E]
12 8 Pepsin (pH 1.3) [^HKR] [^P] [^R] [FL] [^P]
13 8 Pepsin (pH 1.3) [^HKR] [^P] [FL] NULL [^P]
14 9 Pepsin (pH>2) [^HKR] [^P] [^R] [FLWY] [^P]
15 9 Pepsin (pH>2) [^HKR] [^P] [FLWY] NULL [^P]
16 10 Proline-endopeptidase [HKR] [P] [^P]
17 11 Proteinase K [AEFILTVWY]
18 12 Staphylococcal Peptidase I [^E] [E]
19 13 Thermolysin [^DE] [AFILMV] [^P]
20 14 Thrombin [G] [R] [G]
21 14 Thrombin [AFGILTVM] [AFGILTVWA] [P] [R] [^DE] [^DE]
22 15 Trypsin [KR] [^P]
23 15 Trypsin [W] [K] [P]
24 15 Trypsin [M] [R] [P]
25 15 Trypsin [CD] [K] [D]
26 15 Trypsin [C] [K] [HY]
27 15 Trypsin [C] [R] [K]
28 15 Trypsin [R] [R] [HR]
Source: PeptideCutter